中国麻风皮肤病杂志 ›› 2024, Vol. 40 ›› Issue (12): 847-850.doi: 10.12144/zgmfskin202412847

• 论著 • 上一篇    下一篇

免疫荧光检测石蜡切片IgA蛋白在诊断疱疹样皮炎和线状IgA大疱性皮病的应用评价

王建文1，2，3,刘永霞1，2,陈祚玺1，2,陈学超1，2,周桂芝1，2,王田宇1，2,陈声利1，2,孙勇虎1，2,张福仁1，2，3   

  1. 1山东第一医科大学附属皮肤病医院，山东济南，250022；2山东省皮肤病性病防治研究所，山东济南，250022；3山东中医药大学，山东济南，250014
  • 出版日期:2024-12-15 发布日期:2024-10-31

Assessment of IgA protein in paraffin sections by immunofluorescence in the diagnosis of dermatitis herpetiformis and linear IgA bullous dermatitis

WANG Jianwen1,2,3, LIU Yongxia1,2, CHEN Zuoxi1,2, CHEN Xuechao1,2, ZHOU Guizhi1,2, WANG Tianyu1,2, CHEN Shengli1,2, SUN Yonghu1,2, ZHANG Furen1,2,3   

  1. 1 Hospital for Skin Diseases, Shandong First Medical University, Jinan 250022, China；2 Shandong Provincial Institute of Dermatology and Venereology, Shandong Academy of Medical Sciences, Jinan 250022, China；3 University of Traditional Chinese Medicine, Jinan 250014, China
  • Online:2024-12-15 Published:2024-10-31

PDF (PC)

78

摘要： 目的：评价直接免疫荧光染色检测石蜡切片IgA抗体对于诊断疱疹样皮炎（DH）和线状IgA大疱性皮病（LABD）的应用价值。方法：选取2019-2024年我院病理诊断的DH和LABD病例，入选实验组病例DH 46例，LABD 32例。采用FITC荧光标记兔抗人IgA抗体进行直接免疫荧光染色检测。结果：46例DH石蜡切片中，40例IgA在真皮乳头为阳性，阳性率86.96%。32例LABD石蜡切片中，20例IgA在表皮基底膜线状沉积为阳性，阳性率62.50%。结论：直接免疫荧光染色检测石蜡切片IgA可以作为辅助诊断DH和LABD的有效方法。

关键词: 疱疹样皮炎, 线状IgA大疱性皮病, 石蜡切片, 免疫荧光, IgA

Abstract: Objective: To evaluate the application value of direct immunofluorescence staining for detecting IgA antibodies in paraffin sections in the diagnosis of dermatitis herpetiformis (DH) and linear IgA bullous dermatosis (LABD). Methods: DH and LABD cases diagnosed pathologically in our hospital from 2019 to 2024 were selected, including 46 cases of DH and 32 cases of LABD. Direct immunofluorescence staining was performed using FITC-labeled rabbit anti-human IgA antibody. Results: Among the 46 paraffin sections of DH patients, IgA was positively deposited in the dermal papillae in 40 of them, with a positivity rate of 86.96%. Among the 32 paraffin sections of LABD patients, IgA was positively deposited linearly in the epidermal basement membrane in 20 of them, with a positivity rate of 62.50%. Conclusion: Direct immunofluorescence staining for detecting IgA in paraffin sections can be used as an effective method for assisting in the diagnosis of DH and LABD.

Key words: dermatitis herpetiformis, linear IgA bullous dermatitis, paraffin section, immunofluorescence, IgA