XBP1在白癜风皮损及应激HaCaT细胞中的表达

中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (5): 261-264.

XBP1在白癜风皮损及应激HaCaT细胞中的表达

李舒丽杨钰琪张伟刚朱冠男高天文李春英

第四军医大学西京皮肤医院，西安，710032

出版日期:2017-05-15 发布日期:2018-12-19
通讯作者: 李春英，E-mail: lichying@fmmu.edu.cn

Expression of XBP1 in vitiligo lesions and stressed keratinocytes

LI Shuli, YANG Yuqi, ZHANG Weigang, ZHU Guannan, GAO Tianwen, LI Chunying.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China

Online:2017-05-15 Published:2018-12-19
Contact: LI Chunying, E-mail: lichying@fmmu.edu.cn

摘要/Abstract

摘要： 目的：检测XBP1在白癜风皮损组织及H2O2诱导的HaCaT细胞中的表达。方法： H2O2处理体外培养的HaCaT细胞。Real time PCR检测白癜风皮损中及应激HaCaT细胞中XBP1的表达；ELISA检测应激HaCaT细胞中特异性抑制剂抑制XBP1活化前后IL-6和IL-8的表达。结果：白癜风皮损和应激HaCaT细胞中XBP1 mRNA显著高于正常对照；应激HaCaT细胞中IL-6和IL-8水平高于空白对照组，抑制XBP1活化后两者分泌减少。结论：XBP1在白癜风皮损组织中显著上调，促进应激的HaCaT细胞分泌IL-6、IL-8。

关键词: XBP1, 角质形成细胞, 白癜风

Abstract: Objective: To detect the expression level of XBP1 in vitiligo lesions and stressed HaCaT cells. Methods: The cultured HaCaT cells were treated by H2O2. The levels of XBP1 mRNA in the lesions of vitiligo and stressed HaCaT cells were detected by Real-time PCR. The level of IL-6 and IL-8 secretion were detected by ELISA in stressed HaCaT cells before and after treatment with XBP1 inhibitors. Results: The levels of the XBP1 mRNA expression in the lesions of vitiligo and stressed HaCaT cells were higher than that in the control group. The levels of IL-6 and IL-8 in the stressed HaCaT cells were higher than those in the normal HaCaT cells and decreased after the activation of XBP1 was inhibited. Conclusion: The expression level of XBP1 is up-regulated and promote the secretion of IL-6 and IL-8 in the stressed keratinocytes.

Key words: XBP1, keratinocytes, vitiligo

李舒丽, 杨钰琪, 张伟刚, 朱冠男, 高天文, 李春英. XBP1在白癜风皮损及应激HaCaT细胞中的表达[J]. 中国麻风皮肤病杂志, 2017, 33(5): 261-264.

LI Shuli, YANG Yuqi, ZHANG Weigang, ZHU Guannan, GAO Tianwen, LI Chunying..

Expression of XBP1 in vitiligo lesions and stressed keratinocytes [J]. China Journal of Leprosy and Skin Diseases, 2017, 33(5): 261-264.

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