实时荧光定量 PCR 在皮肤结核诊断中的应用

doi:10.12144/zgmfskin201904215

China Journal of Leprosy and Skin Diseases ›› 2019, Vol. 35 ›› Issue (4): 215-218.doi: 10.12144/zgmfskin201904215

The role of Real-time PCR in the diagnosis of cutaneous tuberculosis

MAN Chunhua^1,2,3,4, MI Zihao^2,3, SUN Lele^2,3, LU Xianmei^2,3, WANG Chuan^2,3, WANG Jianwen^2,3, CHEN Xuechao^2,3, LIU Yongxia^2,3, CHEN Shengli^2,3, LIU Hong^2,3, ZHANG Furen^1,2,3

1. School of Medicine and Life Sciences，University of Jinan－Shandong Academy of Medical Sciences，Jinan 250062，China; 2．Shandong Provincial Institute of Dermatology and Venereology，Jinan 250022，China; 3． Shandong Provincial Hospital for Skin Diseses，Jinan 250022，China；4. Department of Dermatology Zoucheng People‘s Hosptal，Zoucheng 273500,China

Online:2019-04-15 Published:2019-05-17
Contact: ZHANG Furen，E-mail: zhangfuren@hotmail.com

Abstract

Abstract: Objective: To compare the sensitivity of Real-time fluorescence quantitative PCR (qPCR) in detecting Mycobacterium tuberculosis (MTB) in cutaneous tuberculosis (CTB) both in paraffin-embedded tissues and fresh tissues，to discuss the feasibility of qPCR detection in paraffin tissues of CTB. Methods: MTB in 6 fresh tissues and 20 paraffin-embedded tissues of CTB were detected by qPCR. Results: The sensitivity rate of fresh tissues and paraffin-embedded tissues were 50% and 35% respectively. Conclusion: QPCR can be applied both in the fresh CTB tissues and paraffin-embedded tissues. The sensitivity of the former is higher than the latter.

Key words: tuberculosis, cutaneous tuberculosis, qPCR

MAN Chunhua, MI Zihao, SUN Lele, LU Xianmei, WANG Chuan, WANG Jianwen, CHEN Xuechao, LIU Yongxia, CHEN Shengli, LIU Hong, ZHANG Furen. The role of Real-time PCR in the diagnosis of cutaneous tuberculosis[J].China Journal of Leprosy and Skin Diseases, 2019, 35(4): 215-218.

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The role of Real-time PCR in the diagnosis of cutaneous tuberculosis

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