中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (7): 388-390.

• 论著 • 上一篇    下一篇

真核细胞翻译起始因子4E在皮肤基底细胞癌中的表达

邓越，刘海东，庄乐，马伟元   

  1. 山东大学齐鲁医院皮肤科，济南，250012
  • 出版日期:2016-07-15 发布日期:2018-12-21
  • 通讯作者: 马伟元，E-mail: dermatologyqlh@163.com

Expression of eIF4E in basal cell carcinoma

DENG Yue, LIU Haidong, ZHUANG Le, MA Weiyuan   

  1. Department of Dermatology, Qilu Hospital of Shandong University, Jinan 250012, China
  • Online:2016-07-15 Published:2018-12-21
  • Contact: MA Weiyuan, E-mail: dermatologyqlh@163.com

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摘要： 目的：检测皮肤基底细胞癌中真核细胞翻译起始因子4E(eIF4E)的表达。方法：采用免疫组化PV-9000法和Real-time PCR法分别检测20例皮肤基底细胞癌及20名健康皮肤组织中eIF4E蛋白及mRNA的表达。结果：基底细胞癌和健康皮肤组织中eIF4E蛋白MOD值分别为(0.198±0.031)和(0.096±0.015)，mRNA相对表达量分别为(2.32±1.00)和(1.24±0.17)，差异具有统计学意义(P<0.05)。结论：eIF4E可能参与皮肤肿瘤的发病过程。

关键词: 基底细胞癌, 真核细胞翻译起始因子4E, 免疫组化, 实时荧光定量PCR

Abstract: Objective:To detect the expression of eukaryotic translation initiation factor 4E (eIF4E) in basal cell carcinoma (BCC). Methods: The protein and mRNA expressions of eIF4E were detected by PV-9000 immunohistochemistry and real-time PCR in 20 patients with basal cell carcinoma and 20 healthy controls. Results:The MODs in BCC and controls were 0.198±0.031 and 0.096±0.015 respectively and the expression levels of mRNA were 2.32±1.00 and 1.24±0.17, with significant differences (P<0.05). Conclusion:eIF4E may involve in the pathogenesis of BCC.

Key words: basal cell carcinoma, eIF4E, immunohistochemistry, RT-PCR