CD8+T细胞对白癜风患者黑素细胞的杀伤机制

中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (6): 341-343.

CD8⁺T细胞对白癜风患者黑素细胞的杀伤机制

马锦媛杨钰琪李舒丽郭伟楠宋璞安亚文高天文李春英

第四军医大学西京皮肤医院，陕西西安，710032

出版日期:2017-06-15 发布日期:2018-12-14
通讯作者: 李春英，E-mail: lichying@fmmu.edu.cn

Mechanism of CD8⁺T cells killing melanocytes in the patients with vitiligo

MA Jinyuan, YANG Yuqi, LI Shuli, GUO Weinan, SONG Pu, AN Yawen, GAO Tianwen, LI Chunying.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University，Xi'an 710032, China

Online:2017-06-15 Published:2018-12-14
Contact: LI Chunying, E-mail: lichying@fmmu.edu.cn

摘要/Abstract

摘要： 目的：明确CD8⁺T细胞对白癜风患者黑素细胞的杀伤机制。方法：分选白癜风患者及正常人外周血CD8⁺T细胞，分别与原代黑素细胞及永生化正常黑素细胞系PIG1及永生化白癜风黑素细胞系PIG3V建立共孵育杀伤模型，采用流式细胞术检测靶细胞被杀伤情况，酶联免疫吸附法(ELISA)检测杀伤环境中IFN-γ、TNF-α、颗粒酶B及穿孔素的分泌水平。结果：白癜风患者外周血CD8⁺T细胞杀伤原代黑素细胞及PIG3V能力，分泌IFN-γ、颗粒酶B及穿孔素水平均高于健康对照，而杀伤PIG1能力和TNF-α水平并无显著差异；白癜风患者CD8⁺T细胞IFN-γ分泌水平与细胞杀伤率呈明显正相关。结论：白癜风患者CD8⁺T细胞杀伤黑素细胞是白癜风发病的重要机制，且该杀伤作用可能依赖IFN-γ、颗粒酶B及穿孔素分泌水平的上调。

关键词: 白癜风, CD8⁺T细胞, 干扰素γ, 颗粒酶B, 穿孔素

Abstract: Objective: To determine the killing mechanism of CD8⁺T cells to melanocytes in the patients with vitiligo. Methods: CD8⁺T cells were obtained from peripheral blood in 8 patients with vitiligo and 8 healthy controls. The primary melanocyte, immortalized normal melanocyte cell line PIG1 and immortalized vitiligo melanocyte cell line PIG3V were co-cultivated with selected CD8⁺T cells respectively. The number of the death of melanocytes killed by CD8⁺T cells was assessed by Flow cytometry. The expression levels of IFN-γ, TNF-α, granzyme B and perforin were measured by ELISA. Results: The number of died primary melanocytes and PIG3V killed by CD8⁺T cells, the expression level of IFN-γ, granzyme B and perforin in the patients with vitiligo was higher than those in controls. There was no significant difference in the number of died PIG1 and the expression level of TNF-α between the patients and healthy controls. There was an obviously positive correlation between the level of IFN-γ secreted by CD8⁺ T cells and the number of died melanocytes killed by CD8⁺T cells. Conclusion: The killing of melanocytes by CD8⁺T cells plays an important role in the development of vitiligo, which probably depends on the increased expression of IFN-γ, granzyme B and perforin.

Key words: vitiligo, CD8⁺T cells, IFN-γ, granzyme B, perforin

马锦媛, 杨钰琪, 李舒丽, 郭伟楠, 宋璞, 安亚文, 高天文, 李春英. CD8⁺T细胞对白癜风患者黑素细胞的杀伤机制[J]. 中国麻风皮肤病杂志, 2017, 33(6): 341-343.

MA Jinyuan, YANG Yuqi, LI Shuli, GUO Weinan, SONG Pu, AN Yawen, GAO Tianwen, LI Chunying.. Mechanism of CD8⁺T cells killing melanocytes in the patients with vitiligo[J]. China Journal of Leprosy and Skin Diseases, 2017, 33(6): 341-343.

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