中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (7): 407-409.

• 论著 • 上一篇    下一篇

银杏叶提取物EGb761对氧化应激下白癜风黑素细胞抗氧化作用的影响

肖茜  崔婷婷  高天文  王琳   

  1. 第四军医大学西京皮肤医院，陕西西安，710032 
  • 出版日期:2017-07-15 发布日期:2018-12-01
  • 通讯作者: 王琳, E-mail: wanglin@fmmu.edu.cn

Effects of ginkgo biloba extract EGb761 on anti-oxidation of melanocytes under oxidative stress in vitiligo

XIAO Qian, CUI Tingting, GAO Tianwen, WANG Lin.    

  1. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
  • Online:2017-07-15 Published:2018-12-01
  • Contact: WANG Lin, E-mail: wanglin@fmmu.edu.cn

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摘要： 目的：明确银杏叶提取物(EGb761)对氧化应激下白癜风黑素细胞抗氧化作用的影响。方法：人正常黑素细胞系PIG1予以H2O2处理建立氧化应激模型，予以不同浓度（50 μg/mL、100 μg/mL、200 μg/mL、300 μg/mL、400 μg/mL）EGb761处理，采用MTT法检测PIG1细胞活力，生物化学方法检测脂质过氧化代谢产物丙二醛（MDA）、乳酸脱氢酶(LDH), 谷胱甘肽过氧化物酶活性及含量，流式细胞仪检测细胞内活性氧（ROS）水平。结果：与模型组比较，EGb761组PIG1细胞活力、SOD、GSH-Px水平增高，ROS、MDA及LDH的表达水平降低。结论：银杏叶提取物EGb761可保护黑素细胞抵抗氧化应激损伤。

关键词: 银杏叶提取物, 白癜风, 氧化应激

Abstract: Objective: To determine the effect of ginkgo biloba extract (EGb761) on the anti-oxidation of melanocytes under oxidative stress in vitiligo. Methods: The normal human melanocyte cell line PIG1 was treated with H2O2 firstly, followed by different concentrations (50μg/mL, 100μg/mL, 200μg/mL, 300 μg/mL, 400 μg/mL) of EGb761. The cell viability was measured by MTT. The levels of lipid peroxide metabolites such as malondialdehyde (MDA), lactic dehydrogenase (LDH) and GSH-Px were measured by biochemical methods. The level of cellular reactive oxygen species (ROS) was detected by flow cytometry. Results: Compared with control group, the expression levels of PIGI cell viability, SOD and GSH-Px were increased, and the expression levels of ROS, MDA and LDH were decreased in EGb761 group. Conclusion: EGb761 has a potential role in protecting melanocytes against H2O2-induced oxidative stress.

Key words: ginkgo biloba extract, vitiligo, oxidative stress