Abstract: Objective: To investigate the prevalence and subtype distribution of human papillomavirus (HPV) infection in patients attending the Shandong Provincial Hospital for Skin Diseases of Shandong First Medical University. Methods: HPV typing was identified by PCR-reverse dot hybridization among 6900 patients admitted to the outpatient department of urinary Infection of our hospital from April 2021 to April 2023 and the data were analyzed by the chi-square test. Results: Of the 6900 samples, 4803 were male and 2097 were female, ranging in age from 1 to 84 years old. 4377 cases were positive for HPV, the positivity rate was 63.43%. The top six HPV types were HPV6 (23.52%), HPV11 (14.65%), HPV16 (8.49%), HPV52 (8.43%), HPV42 (7.30%), and HPV51 (6.28%). There were 2154 cases of HPV single infection (31.22%), and 2223 cases of polyinfection (32.22%). The HPV infection rates in different age groups of <20, 20-30, 31-40, 41-50 and >50 years were 71.78%, 64.84%, 60.11%, 61.51%, and 68.30%, respectively, and the peak of HPV infection occurred in men below 20 years and above 50 years, and in women below 30 years and above 50 years. There were significant differences in HPV infection rates between men and women at different ages (Ps<0.05). Conclusion: HPV high-risk type infection rate was significantly higher in female than in male in this study, while HPV low-risk type infection rate in female was lower than in male. HPV infection rate in male and female showed a U-shaped distribution with age.

Key words: human papillomavirus, condyloma acuminatum, genotypes