China Journal of Leprosy and Skin Diseases ›› 2024, Vol. 40 ›› Issue (12): 847-850.doi: 10.12144/zgmfskin202412847

• Original Articles • Previous Articles     Next Articles

Assessment of IgA protein in paraffin sections by immunofluorescence in the diagnosis of dermatitis herpetiformis and linear IgA bullous dermatitis

WANG Jianwen1,2,3, LIU Yongxia1,2, CHEN Zuoxi1,2, CHEN Xuechao1,2, ZHOU Guizhi1,2, WANG Tianyu1,2, CHEN Shengli1,2, SUN Yonghu1,2, ZHANG Furen1,2,3   

  1. 1 Hospital for Skin Diseases, Shandong First Medical University, Jinan 250022, China；2 Shandong Provincial Institute of Dermatology and Venereology, Shandong Academy of Medical Sciences, Jinan 250022, China；3 University of Traditional Chinese Medicine, Jinan 250014, China
  • Online:2024-12-15 Published:2024-10-31

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Abstract: Objective: To evaluate the application value of direct immunofluorescence staining for detecting IgA antibodies in paraffin sections in the diagnosis of dermatitis herpetiformis (DH) and linear IgA bullous dermatosis (LABD). Methods: DH and LABD cases diagnosed pathologically in our hospital from 2019 to 2024 were selected, including 46 cases of DH and 32 cases of LABD. Direct immunofluorescence staining was performed using FITC-labeled rabbit anti-human IgA antibody. Results: Among the 46 paraffin sections of DH patients, IgA was positively deposited in the dermal papillae in 40 of them, with a positivity rate of 86.96%. Among the 32 paraffin sections of LABD patients, IgA was positively deposited linearly in the epidermal basement membrane in 20 of them, with a positivity rate of 62.50%. Conclusion: Direct immunofluorescence staining for detecting IgA in paraffin sections can be used as an effective method for assisting in the diagnosis of DH and LABD.

Key words: dermatitis herpetiformis, linear IgA bullous dermatitis, paraffin section, immunofluorescence, IgA