Abstract: Objective: To determine the impact of 5-ALA-PDT on the HPV11.HaCaT cell model (HaCaT keratinocytes harboring human papillomavirus type 11 episome). Methods: Cultured HPV11.HaCaT cells were treated with 5-ALA (0.375、0.75、1.5、3mM), red light irradiation (10、20、40J/cm2), or different concentration of 5-ALA combined with different dose of red light irradiation. The effects on cells proliferation were examined by MTT assay. The alterations of HPV11 E5, E6 and E7 mRNA expression level were examined by real-time fluorescence quantitative PCR. Results: When 5-ALA concentration was higher than 0.75mM and combined with 20 or 40J/cm2 red light irradiation, the survival rate of the cells was less than 50% (P<0.01). When 0.375mM 5-ALA combined with 20J/cm2 red light irradiation, the expression of HaCaT keratinocytes was significantly reduced, with relative expression rates of 0.28 and 0.26 (P<0.01). 0.75mM 5-ALA combined with 20J/cm2 red light irradiation did not inhibit the expression of HPV11 E6 and E7 mRNA, with relative expression rates of 0.85 and 1.46 (P>0.05). However, the two kinds of treatment mentioned above did not affect the expression of HPV11 E5 mRNA. Conclusion: 5-ALA-PDT can effectively impact on the HPV11. HaCaT cell model, such as the proliferation of the HPV11. HaCaT cells and the expression of HPV11 E6 and E7 mRNA.

Key words: HPV11.HaCaT, condyloma acuminatum, Photodynamic therapy, cell proliferation, early genes