China Journal of Leprosy and Skin Diseases ›› 2018, Vol. 34 ›› Issue (8): 449-452.

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The expression of mTORC1 pathway in murine model of systemic lupus erythematosus

XIE Shuangde1，WEI Shanshan2, ZHANG Huanian1, WANG Guisong1, PENG Xuebiao1, Zhao Ke3, LI Junhao1，LAI Kuan1   

  1. 1.Departmen of Dematology,Nanfang Hospital，Southern Medical University,Guangzhou 510515，China;2.Departmen of Dematology,Zhujiang Hospital,Southern Medical University，Guangzhou 510282，China;3.Department of Hematology,Nanfang Hospital，Southern Medical University，Guangzhou 510515, China
  • Online:2018-08-15 Published:2018-12-11
  • Contact: LAI Kuan, E-mail:kuan_lai@163.com

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Abstract: Objective: To detect the level of mTORC1 pathway related factors in the murine model of systemic lupus erythematosus (SLE) and to explore its effect in the development of SLE. Methods: There were 6 B6.MRL/lpr lupus mice in the experimental group and 6 C57 mice in the control group. The levels of Akt, mTOR, p70S6K, IL-17 mRNA and protein in kidney were detected by RT-PCR and western blot. The percentage of Th17 lymphocytes in CD4+T cells in spleen was detected by flow cytometry. Results: The mRNA and phosphoprotein of Akt, mTOR, p70S6K and IL-17 in the experimental group were higher than those in the control group. The percentage of Th17 lymphocytes in CD4+T cells in the experimental group was higher than that in the control group. Conclusion: The activation of mTORC1(Akt-mTOR-p70S6K) pathway may play an important role in the development of SLE.

Key words: systemic lupus erythematosus, mTOR, p70S6K, Th17