Abstract: Objective: To identify the normal range of fungus cloning in different body site of healthy skin and lesions of inflammatory dermatosis. Methods: The patients with psoriasis, eczema, atopic dermatosis and pityriasis rosea were collected. The fungus in different sites including sebaceous sites (face, chest and back), moist sites (bend of elbow, back of knee and groin), dry sites (volar forearm and palm) and scalp of patients and healthy controls were detected by fluorescence staining, and the number of fungi in 30 microscopic areas were counted and statistically analyzed using R 3.5.3. Results: Four hundred and fifty seven patients and 888 controls were collected. The positivity rate of fungi on the scalp and sebaceous sites of healthy volunteers were 95% and 96% highest, which on the plantar was the lowest (44.29 %). The positivity rate of fungi in the sebaceous or oily site of patients with eczema, psoriasis and pityriasis rosea were 77.05%, 66.67% and 91.42%, which were higher than that in the dry site (58.93%，47.62%，57.89%). The positivity rate of fungi in the scalp, sebaceous sites, dry sites in the healthy controls were higher than that in the corresponding sites of the patients with inflammatory dermatosis, with significant differences (Ps<0.05). There was a signitficant different of the amount of fungi among the sebaceous sites, moist sites, dry sites and scalpe in the healthy controls (P<0.05). The number of spores in the sebum area was the highest in the 18-44 age group. Conclusion: The spores and fungal infection rates in different sites are different. The number of fungal spores in the lesions of the patients with psoriasis, eczema, atopic dermatosis and pityriasis rosea were less than that in healthy controls.

Key words: fluorescent staining, fungus, skin, psoriasis, atopic dermatosis (AD), eczema, pityriasis rosea (PR)