中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (4): 212-216.doi: 10.12144/zgmfskin202104212

• 论著 • 上一篇    下一篇

RRS1对皮肤鳞状细胞癌细胞增殖、转移和侵袭的影响

潘展砚,吴琼,王岚琦,胡婷婷,鞠强   

  1. 上海交通大学医学院附属仁济医院皮肤科，上海，200127
  • 出版日期:2021-04-15 发布日期:2021-04-07
  • 通讯作者: 鞠强，E-mail: Qiangju401@sina.com

Effect of RRS1 on the proliferation, metastasis and invasion of cutaneous squamous cell carcinoma cells

PAN Zhanyan, WU Qiong, WANG Lanqi, HU Tingting, JU Qiang   

  1. Department of Dermatology, Ren ji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200127, China
  • Online:2021-04-15 Published:2021-04-07
  • Contact: JU Qiang, E-mail: Qiangju401@sina.com

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摘要： 目的：明确RRS1(核糖体合成调节因子1)对皮肤鳞状细胞癌(cSCC)细胞增殖、转移和侵袭的影响。方法：通过免疫组化和GEO数据库明确RRS1在cSCC与正常皮肤中表达的差异。慢病毒感染法建立RRS1敲低的cSCC细胞系SCL-1细胞。MTT实验、Celigo划痕实验、Transwell实验研究RRS1敲低后SCL-1细胞增殖、转移和侵袭性的变化。QRT-PCR法检测增殖、转移和侵袭相关基因表达。结果：RRS1在cSCC细胞中表达量明显高于正常皮肤，RRS1敲低后SCL-1细胞增殖、转移和侵袭性均降低。RRS1敲低后，增殖相关基因FGF2，AREG，cyclin E1显著降低。转移和侵袭相关基因VIM，CXCL1，CXCL3，CXCL8，MMP1表达显著降低。 结论：RRS1可能参与了cSCC细胞的增殖、转移和侵袭过程。

关键词: 核糖体合成调节因子1, 皮肤鳞状细胞癌

Abstract: Objective: To determine the effect of ribosome synthesis regulator 1 (RRS1) on the proliferation, metastasis and invasion of cutaneous squamous cell carcinoma (cSCC) cells. Methods: The difference of RRS1 expression in cSCC and normal skin was confirmed by immunohistochemical method and GEO database. SCL-1 cells with RRS1 knockdown were established by lentivirus infection. MTT, Celigo scratch, and Transwell were used to study the proliferation, metastasis and invasiveness of SCL-1 cells after RRS1 knockdown. The expression of genes related to proliferation, metastasis and invasion was detected by QRT-PCR. Results: The expression of RRS1 in cSCC cells was significantly higher than that in normal skin. The proliferation, metastasis and invasiveness of SCL-1 cells were reduced after RRS1 knockdown. After knocking down RRS1, the proliferation-related genes FGF2, AREG, and cyclin E1 were significantly reduced. Metastasis and invasion-related genes VIM, CXCL1, CXCL3, CXCL8, and MMP1 are significantly reduced. Conclusion: RRS1 may participate in the cell proliferation, metastasis and invasion of cSCC. 

Key words: RRS1, cSCC