中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (11): 665-668.

• 论著 • 上一篇    下一篇

朗格汉斯细胞表面分子与尖锐湿疣复发的相关性

范向华，缪 旭，林 桦，施 健   

  1. 南通市第一人民医院皮肤科，江苏南通，226001
  • 出版日期:2016-11-15 发布日期:2018-12-20
  • 通讯作者: 缪旭，E-mail: mx730129@163.com

Relationship between the surface molecules of Langerhans cells and recurrence of condyloma acuminatum

Fan Xianghua，MIAO Xu，Lin Hua, SHI Jian   

  1. Department of Dermatology, Nantong First People's Hospital, Nantong 226001, China
  • Online:2016-11-15 Published:2018-12-20
  • Contact: MIAO Xu, E-mail: mx730129@163.com

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摘要： 目的：确定朗格汉斯细胞（Langerhans cells，LC）表面分子与尖锐湿疣（condyloma acuminatum，CA）复发的相关性。方法：采用免疫组化方法对正常包皮、CA初发和复发患者皮损组织中LC进行CD1a、S100、HLA-DR标记，并对其表达强度进行半定量分析。结果：正常对照组、CA初发组及CA复发组CD1a免疫组化染色IOD值分别为7644.84±2865.10、4075.62±2410.39和2308.08±1256.34；S100分别为4400.34±2962.29、1815.78±1013.35及1414.46±1337.13，CA组织中LC的CD1a及S100分子表达较正常组织显著下降（P<0.05）, 在复发组尤为明显。HLA-DR IOD值分别为583.91±592.85、2262.87±2983.09和1191.75±1698.36。HLA-DR的表达在CA初发组和复发组均强于正常组，但复发组较初发组下降。结论：HPV感染人体后，会导致HPV感染的免疫逃避，造成CA的持续感染和易复发。

关键词: 尖锐湿疣, 人乳头瘤病毒, 免疫组化, 朗格汉斯细胞

Abstract: Objective: To determine the relationship between the surface molecules of langerhans cells (LC) and recurrence of condyloma acuminatum (CA). Methods: The expression of CD1a, S100 and HLA-DR of LC was measured by immunohistochemical technique, and their semi-quantities were analyzed by graphic software in skin samples of control group, primary CA group and recurrent CA group. Results: In control group, primary CA group and recurrent CA group, the IOD of immunohistochemical staining of CD1a were 7644.84±2865.10, 4075.62±2410.39 and 2308.08±1256.34; The IOD of S100 were 4400.34±2962.29, 1815.78±1013.35 and 1414.46±1337.13. The staining intensity of CD1a and S100 in CA decreased significantly when compared with control group (P<0.05), with an even more decrease in the recurrent CA group. The IOD of HLA-DR were 583.91±592.85, 2262.87±2983.09 and 1191.75±1698.36 in the three groups respectively. The staining intensity of HLA-DR was increased in primary and recurrent CA groups, when compared with control group, but the expression in recurrent group decreased when compared with primary group. Conclusion: After infection of HPV, immune escape will occure and lead to the persistence and recurrence of CA.

Key words: Condyloma acuminatum, human papillomavirus, immunohistochemical staining, langerhans cells