中国麻风皮肤病杂志 ›› 2026, Vol. 42 ›› Issue (3): 158-162.doi: 10.12144/zgmfskin202603158

• 论著 • 上一篇    下一篇

柠檬烯治疗大鼠接触性皮炎模型疗效评价

赵好,王胜兰,陈佳铭,杨晓艳,李玲双,张龙飞,孙东杰   

  1. 昆明医科大学第一附属医院皮肤科，云南昆明，650032
  • 出版日期:2026-03-15 发布日期:2026-03-09

Evaluation of the therapeutic effect of limonene on a rat model contact dermatitis

ZHAO Hao, WANG Shenglan, CHEN Jiaming, YANG Xiaoyan, LI Lingshuang, ZHANG Longfei, SUN Dongjie   

  1. Department of Dermatology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
  • Online:2026-03-15 Published:2026-03-09

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摘要： 目的：评价柠檬烯对大鼠接触性皮炎的治疗效果。方法：建立大鼠的接触性皮炎模型，36只大鼠随机分为阴性对照组、接触性皮炎模型组、低柠檬烯剂量组（100 mg/kg）、中柠檬烯剂量组（200 mg/kg）、高柠檬烯剂量组（400 mg/kg）及阳性对照地塞米松组（0.41 mg/kg），每组6只，连续给药10天。给药前后进行皮损评分、组织病理学及血清IL-2、IL-4、IL-6和IFN-γ水平检测。结果：柠檬烯组大鼠皮损评分，表皮增生、毛细血管扩张以及炎性细胞浸润及血清IL-2、IL-4、IL-6和IFN-γ水平均明显低于模型组，且高剂量柠檬烯最显著。柠檬烯高剂量组皮损改善和炎性指标改变情况优于地塞米松组（P＜0.05）。结论：柠檬烯可明显改善接触性皮炎皮损并降低炎性因子水平。

关键词: 柠檬烯, 接触性皮炎, 细胞因子

Abstract: Objective: Investigate the therapeutic effects of limonene on contact dermatitis in rats. Methods: Establish a rat model of contact dermatitis, 36 rats were randomly divided into 6 groups with 6 rats in each group: blank group, contact dermatitis model group, low-dose limonene group (100 mg/kg), the medium-dose limonene group (200 mg/kg), the high-dose limonene group (400 mg/kg), and the positive control dexamethasone group (0.41 mg/kg). All groups were treated continuously for 10 days. Before and after treatment, lesion scoring, histopathological examination, and detection of serum levels of IL-2, IL-4, IL-6 and IFN-γ were performed. Results: Compared with the model group, the limonene-treated groups showed significantly lower lesion scores, reduced epidermal hyperplasia, capillary dilation, inflammatory cell infiltration, as well as decreased serum levels of IL-2, IL-4, IL-6 and IFN-γ, with the high-dose limonene group exhibiting the most prominent effect. Moreover, the high-dose limonene group showed superior improvements in skin lesions and inflammatory indicators compared with the dexamethasone group (P＜0.05). Conclusion: Limonene can significantly ameliorate lesions of contact dermatitis and reduce the levels of inflammatory factors.

Key words: limonene, contact dermatitis, cytokine