中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (4): 207-210.

• 论著 • 上一篇    下一篇

NB-UVB对HaCaT细胞CCL22表达的影响

王延婷 李舒丽 杨钰琪 高天文 李春英   

  1. 第四军医大学西京皮肤医院，陕西西安，710032
  • 出版日期:2016-04-15 发布日期:2018-12-24
  • 通讯作者: 李春英，E-mail:lichying@fmmu.edu.cn?

Effects of NB-UVB on the expression of CCL22 in HaCaT cells

WANG Yanting, LI Shuli, YANG Yuqi, GAO Tianwen, LI Chunying.   

  1. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China.
  • Online:2016-04-15 Published:2018-12-24
  • Contact: LI Chunying, E-mail:lichying@fmmu.edu.cn

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摘要： 目的：明确NB-UVB对HaCaT细胞CCL22的影响。方法：常规培养后的HaCaT细胞分为两组，一组给予不同剂量NB-UVB (0、100、200、400 mJ/cm2)照射；一组给予NF-κB抑制剂PDTC（1、12.5、25 μmol/L）预处理后再进行NB-UVB照射，采用Real-time PCR及ELISA检测CCL22表达；采用Western blot方法检测NF-κB p65磷酸化水平。结果：CCL22表达和NF-κB p65磷酸化的水平随着NB-UVB照射剂量增强而上调；PDTC处理后，NB-UVB诱导的角质形成细胞CCL22表达及和NF-κB p65磷酸化水平较直接给予NB-UVB明显下调，且PDTC浓度越高越明显。 结论：NB-UVB照射可能通过激活NF-κB信号通路促进角质形成细胞CCL22表达及分泌。

关键词: 窄谱中波紫外线, 角质形成细胞, CCL22

Abstract: Objective: To determine the effects of NB-UVB on the expression of CCL22 from HaCaT cells. Methods: The cultrued HaCaT cells were divided into two groups. The HaCaT cells in one group were treated with different dose of NB-UVB (0、100、200、400 mJ/cm2) and those in the second group were treated with NB-UVB (400 mJ/cm2) after the pretreatment with PDTC. The expression of CCL22 was detected by Real-time PCR and ELISA. The level of NF-κB p65 phosphorylation was detected by western blot. Results: The expression of CCL22 and the level of NF-κB p65 phosphorylation in HaCaT cells treated with NB-UVB were increased as the dose of of NB-UVB was increasing. The expression of CCL22 and the level of NF-κB p65 phosphorylation in HaCaT cells pretreated by PDTC were lower than those treated with NB-UVB only and the higher the concentration of PDTC, the effects were more obvious. Conclusion: The CCL22 expression induced by NB-UVB in keratinocytes may through activation of NF-κB pathway.

Key words: NB-UVB, keratinocytes, CCL22