China Journal of Leprosy and Skin Diseases ›› 2020, Vol. 36 ›› Issue (7): 394-399.doi: 10.12144/zgmfskin202007394

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The effect of paeoniflorin on inflammatory cytokines in macrophages

LIU Zijing1, ZHANG Jiawen1, JIANG Peiyu1, YIN Zhi1, LIU Yunyi1, LIU Yixuan1, WANG Xiaoyan1, LIU Wentao2, XU Yang1   

  1. 1 Department of Dermatology, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China; 
    2 Department of Pharmacology, School of Basic Medical Sciences, Nanjing 211100, China
  • Online:2020-07-02 Published:2020-07-02
  • Contact: XU Yang, E-mail: cardioos@163.com; LIU Wentao, E-mail: painresearch@njmu.edu.cn

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Abstract: Objective: To determine the effect of paeoniflorin (PF) on inflammation cytokines in macrophages induced by LPS. Methods: Mouse mononuclear cell line Raw 264.7 cells were cultured in vitro，and then, were divided into LPS group, LPS+PF group and blank control group. The level of LL37, SOCS3, ASK1, p38 and TLR2 was detected by qRT-PCR and Western blot. Results: LPS induced Raw 264.7 cells activation and inflammatory response. Compared to the blank control group, the level of TLR2, ASK1 and p38 increased in the LPS group, but SOCS3 decreased. Compared to LPS group, the level of TLR2, ASK1 and p38 decreased in the LPS+PF group, but SOCS3 increased. Conclusion: PF can inhibit the inflammation reaction in macrophage.

Key words: osacea, paeoniflorin, macrophage