Abstract: Objective: To analyze the diference of skin and intestinal microflora between psoriasis mice and control group based on 16S rRNA sequencing, and explore the key microflora of psoriasis. Methods: Twelve mice were randomly divided into experimental group and control group. Skin and fecal samples were collected and detected by 16S rRNA sequencing. Results: Compared with the control group, in the experimental group, the relative abundance of bacteroidota, coriobacteriia, lachnospirales, rikenellaceae decreased in the skin flora. Nevertheless, the relative abundance of firmicutes, bacilli, staphylococcales increased in the skin flora. And the relative abundance of bacteroidota, alpha-proteobacteria, lactobacillales decreased in the intestinal flora. But the relative abundance of campilobacterota, clostridia, and lachnospirales increased in the intestinal flora. LefSe analysis showed that coriobacteriia, bacteroidia, lactobacillales, and staphylococcales played a major role in the skin microbiota of mice, and bacteroidia, clostridia, enterobacterales, and lachnospirales played a major role in the gut microbiota of mice. The results of MetagenomeSeq analysis showed that the species composition and function of the skin and gut microbiota in the two groups were different at the phylum, class, order, family, genus, and species levels, with significant differences. Conclusion: The immune system of psoriatic mice is involved, the skin and intestinal barrier function is damaged, and the abundance of bacterial flora is changed, which further verifies the correlation of gut-immune-skin axis.

Key words: psoriasis, skin microbiota, gut microbiota, 16S rRNA