Abstract: Objective: To determine the effect of hydrogen (H2) on the hyperproliferation of HaCaT keratinocytes and the expression of inflammatory factors, and to explore the possible mechanism of hydrogen in the treatment of psoriasis. Methods: Hyperproliferation of HaCaT keratinocytes was induced by M5 (IL-17A, IL-22, tumor inhibin -M, IL-1α And TNF-α）and hydrogen intervention was conducted through hydrogen cell incubator. HaCaT cells were divided into Control group (without any intervention), Model group (treated by 2.5 ng/mL M5), H2 group (treated by 2.5 ng/mL M5 and 25% hydrogen treatment), and Acitretin group (treated by 2.5 ng/mL M5, 10 μMol/L of acitretin A), after 72 hours, the cell viability was determined by CCK-8 method, and the cell proliferation marker KRT6 and inflammatory factor IL-1β, IL-6,TNF-α mRNA expression level were detected by qRT-PCR. Results: CCK-8 assay showed that different concentrations of hydrogen had no effect on normal cell growth. Hydrogen intervention at 25% concentration can significantly inhibit the enhancement of cell viability induced by M5, and reduced the level of KRT6 and IL-1β, IL-6, and NF-αmRNA expression. Conclusion: Hydrogen intervention can inhibit the over proliferation of HaCaT cells induced by M5 and reduce the expression of inflammatory factors.

Key words: hydrogen, HaCaT cells, proliferation, inflammatory cytokines, psoriasis