China Journal of Leprosy and Skin Diseases ›› 2018, Vol. 34 ›› Issue (1): 4-7.

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Detection of PGL-I antibody in 59 patients with leprosy and 87 household contacts by lateral flow immuno-sandwich assay using up-converting phosphor reporter particles

ZHANG Xinhui1,YANG Lili1, YANG Qin2, ZHAO Tingfang3, YANG Changming3, DAI Qian4, YUAN Kang4, WENG Xiaoman5, WEN Yan5, Anouk.van Hooij6, Paul Corstjens6 , Annemieke Geluk6, LAN Yafang7, FU Yun7, LI Jinlan1.   

  1. 1、Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China； 2、Guiyang Center for Disease Control and Prevention,Guiyang, Guizhou 550002, China； 3、Qianxinan Center for Disease Control and Prevention, Xingyi, Guizhou 562400, China； 4、Anshun Center for Disease Control and Prevention,Anshun, Guizhou 561000, China； 5、Beijing Friendship Hospital affiliated to Capital Medical University, Beijing 100050, China； 6、Institute of Infectious Diseases, Leiden University Medical Center, Holland; 7.Guizhou Medical University, Guiyang, Guizhou 550002 , China
  • Online:2018-01-15 Published:2018-12-04
  • Contact: LI Jinlan，E-mail:740820442@qq.com

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Abstract: Objective: To evaluate the value of lateral flow immuno-sandwich assay using up-converting phosphor reporter particles (UCP-LFA) in predicting the risk of leprosy contacts by detecting PGL-I antibody. Methods: The PGL-I IgM level were measured by UCP-LFA in 59 patients with leprosy, 87 household contacts and 55 healthy controls in Guizhou. Data statistics were analyzed by using SPSS 20.0 for chi-square test. The?critical value was identified through receiver operating characteristic curve. Results: The positivity rate of anti-PGL-I IgM in patients, contacts and healthy controls were 81.4%, 5.7% and 1.8%, with significant differences between them (χ2 =126.47，P<0.005). The positivity rates of anti-PGL-I IgM in patients with paucibacillary leprosy (PB) and multibacillary (MB) were 40% and 89%, with a significant difference (χ2=13.386，P<0.005).The positivity rates of anti-PGL-I IgM in patients with BI+ and BI- were 83% and 0%, with a significant difference (χ2 =17.560，P<0.005). Conclusion: The PGL-1 IgM antibody can predict the risk of leprosy.

Key words: UCP-LFA, leprosy