中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (1): 8-11.

• 论著 • 上一篇    下一篇

白癜风免疫微环境对黑素细胞CXCL10表达的影响

李博为 李舒丽 杨钰琪 张伟刚 刘玲 高天文 李春英   

  1. 第四军医大学西京皮肤医院，陕西西安，710032
  • 出版日期:2017-01-15 发布日期:2018-12-10
  • 通讯作者: 李春英，E-mail: lichying@fmmu.edu.cn

Effects of immune microenvironment on the expression of CXCL10 in melanocytes of vitiligo

LI Bowei, LI Shuli, YANG Yuqi, ZHANG Weigang, LIU Ling, GAO Tianwen, LI Chunying   

  1. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China

  • Online:2017-01-15 Published:2018-12-10
  • Contact: LI Chunying, E-mail: lichying@fmmu.edu.cn

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摘要： 目的：明确白癜风免疫微环境对黑素细胞CXCL10表达的影响。方法：采用qRT-PCR检测进展期白癜风患者皮损组织中的细胞因子；采用qRT-PCR及ELISA检测白癜风免疫微环境中上调的细胞因子对黑素细胞系PIG1 CXCL10表达和分泌。结果：进展期白癜风患者皮损组织中IFN-γ、TNF-α和IL-1β mRNA表达上调；三者联合刺激显著上调PIG1细胞CXCL10 mRNA的表达以及蛋白分泌，并呈现时间依赖性。结论：白癜风局部免疫微环境促进黑素细胞表达及分泌CXCL10，可能参与白癜风发病。

关键词: 白癜风, 免疫微环境, 黑素细胞, 趋化因子CXC配体10

Abstract: Objective: To determine the effects of immune microenvironment on the expression of CXCL10 in melanocytes of vitiligo. Methods: Quantitative Real-Time PCR was performed to detect the expression of cytokines in skin lesions from progressive vitiligo patients. And then, a melanocyte cell line-PIG1 was stimulated by the cytokines that were up-regulated in vitiligo lesions and to measure the expression and secretion of CXCL10 in PIG1 cells. Results: The mRNA level of IFN-γ, TNF-α and IL-1β were up-regulated in the skin lesions of progressive vitiligo patients. When a combined stimulation with the three cytokines, the expression and secretion of CXCL10 in PIG1 cells were up-regulated significantly, in a time-dependent manner. Conclusion: Immune microenvironment in vitiligo can promote the expression and secretion of CXCL10 in melanocytes, which may contribute to the onset of vitiligo.

Key words: vitiligo, immune microenvironment, melanocytes, CXCL10