ALA-PDT,皮肤鳞状细胞癌,A431细胞,增殖 ," /> ALA-PDT,皮肤鳞状细胞癌,A431细胞,增殖 ,"/> ALA-PDT,cutaneous squamous cell carcinoma,A431 cell,proliferatio ,"/> <p class="MsoNormal"> <span>不同剂量</span>ALA-PDT<span>对皮肤鳞状细胞癌</span><span>A431</span><span>细胞增殖的抑制作用</span>

中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (4): 193-195.

• 论著 •    下一篇

不同剂量ALA-PDT对皮肤鳞状细胞癌A431细胞增殖的抑制作用

张鑫1,2  郝玉琴1   

  1. 1内蒙古医科大学第三附属医院（内蒙古包钢医院）皮肤科，包头市，014010

    2内蒙古医科大学在读研究生，呼和浩特，010000

  • 出版日期:2017-04-15 发布日期:2018-12-19
  • 通讯作者: 郝玉琴，E-mail: haoyuqin0472@163.com

Inhibition of ALA-PDT on A431 cells in cutaneous squamous cell carcinoma

ZHANG Xin1,2, HAO Yuqin1   

  1. 1. Department of Dermatology, the Third Affiliated Hospital, Inner Mongolia Medical University, Baotou 014010, China; 2. Graduate Student in Inner Mongolia Medical University, Hohehot 010010, China

  • Online:2017-04-15 Published:2018-12-19
  • Contact: HAO Yuqin, E-mail: haoyuqin0472@163.com

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摘要：

目的：确定5-氨基酮戊酸-光动力疗法对皮肤鳞状细胞癌A431细胞抑制的最佳剂量。方法：体外培养人皮肤鳞状细胞癌A431细胞株，应用MTT法检测不同ALA浓度(0.5、1、2 mmol/L)、不同PDT光照剂量(5、10、20、40 J/cm2)下A431细胞的增殖水平。结果：当ALA浓度为0.5 mmol/L时，光照剂量为5、10、20、40 J/cm2对A431细胞的抑制率分别为6%、10%、15%、18%；当ALA浓度为1 mmol/L时，光照剂量为5、10、20、40 J/cm2对A431细胞的抑制率分别为30%、52%、80%、82%；当ALA浓度为2 mmol/L时，光照剂量为5、10、20、40 J/cm2对A431细胞的抑制率分别为31%、54%、81%、82%。结论：ALA在浓度为1 mmol/L,光照剂量为20 J/cm2时，ALA-PDT对A431细胞增殖抑制效果达到最佳

关键词: ALA-PDT')">

ALA-PDT, 皮肤鳞状细胞癌, A431细胞, 增殖

Abstract:

Objective: To determine the optimal dose of ALA-PDT on the inhibition of A431 cells proliferation in cutaneous squamous cell carcinoma. Methods: A431 cells were cultured firstly in vitro, and then, were treated with different drug concentration (0.5, 1 and 2 mmol/L) and different dose of PDT (5, 10, 20 and 40 J/cm2). The proliferation of treated A431 cells was detected by MTT. Results: The inhibition rates of A431 cells treated under the condition of 0.5 mmol/L ALA combined with 5, 10, 20 and 40 J/cm2 PDT were 6%, 10%, 15% and 18% respectively. The inhibition rates of A431 cells treated with 1 mmol/L ALA combined with PDT of 5, 10, 20 and 40 J/cm2 PDT were 30%, 52%, 80% and 82% respectively. The inhibition rates of A431 cells treated with 2 mmol/L ALA combined with 5, 10, 20 and 40 J/cm2 PDT were 31%, 54%, 81% and 82% respectively. Conclusion: The highest inhibition rate of PDT on A431 cell proliferation is 1mmol/L ALA and 20 J/cm2 PDT

Key words: ALA-PDT')">

ALA-PDT, cutaneous squamous cell carcinoma, A431 cell, proliferatio