Abstract: Objective: To determine the effect of ginkgo biloba extract (EGb761) on the anti-oxidation of melanocytes under oxidative stress in vitiligo. Methods: The normal human melanocyte cell line PIG1 was treated with H₂O₂ firstly, followed by different concentrations (50μg/mL, 100μg/mL, 200μg/mL, 300 μg/mL, 400 μg/mL) of EGb761. The cell viability was measured by MTT. The levels of lipid peroxide metabolites such as malondialdehyde (MDA), lactic dehydrogenase (LDH) and GSH-Px were measured by biochemical methods. The level of cellular reactive oxygen species (ROS) was detected by flow cytometry. Results: Compared with control group, the expression levels of PIGI cell viability, SOD and GSH-Px were increased, and the expression levels of ROS, MDA and LDH were decreased in EGb761 group. Conclusion: EGb761 has a potential role in protecting melanocytes against H₂O₂-induced oxidative stress.

Key words: ginkgo biloba extract, vitiligo, oxidative stress