中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (8): 477-480.

• 论著 • 上一篇    下一篇

氧化苦参碱对皮肤鳞状细胞癌Colo-16细胞株增殖及对EGFR表达的影响

张青松1  陈旭2  黄丹2  顾恒2   

  1. 1常熟市中医院皮肤科,常熟，215500
    2中国医学科学院北京协和医学院皮肤病研究所,南京，210042
  • 出版日期:2017-08-15 发布日期:2018-12-05
  • 通讯作者: 顾恒，E-mail：guheng@aliyun.com

Effects of oxymatrine on the proliferation of cutaneous squamous cell carcinoma Colo-16 cell and the expression of EGFR

ZHANG Qingsong1, CHEN Xu2, HUANG Dan2, GU Heng2.   

  1. 1. Department of Dermatology, Traditional Chinese Medical Hospital of Changshu City, Changshu 215500, China; 2. Department of Physical Therapy, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College．Nanjing 210042, China
  • Online:2017-08-15 Published:2018-12-05
  • Contact: Gu Heng, E-mail: guheng@aliyun.com

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摘要： 目的：明确氧化苦参碱对皮肤鳞状细胞癌(cSCC)Colo-16细胞株增殖及对EGFR表达的影响。方法：体外培养Colo-16细胞株，分为空白对照组、5%葡萄糖溶剂对照组、不同浓度氧化苦参碱(0.025 mg/mL、0.05 mg/mL、0.1 mg/mL、0.5 mg/mL、1 mg/mL、2 mg/mL)组。 MTT法测定各组Colo细胞株增殖情况，Western blot印迹法测定各组表皮生长因子受体(EGFR)及磷酸化表皮生长因子受体(pEGFR)的表达。结果：氧化苦参碱的各浓度组在24 h、48 h、72 h OD值均低于空白对照组及溶剂对照组，差异具有统计学意义(P＜0.05)。氧化苦参碱各浓度组的OD值无显著差异(P>0.05)。不同浓度组与空白对照组相比及各不同浓度组之间，EGFR及磷酸化EGFR的表达差异均无统计学意义(P>0.05)。结论：氧化苦参碱在较低的浓度即有抑制Colo-16细胞株增殖的作用，而提高浓度不能增强抑制作用。氧化苦参碱并非通过抑制EGFR的表达来影响Colo-16细胞的增殖。

关键词: 氧化苦参碱, 皮肤鳞状细胞癌, 增殖

Abstract: Objective: To determine the effects of oxymatrine on the proliferation of cutaneous squamous cell carcinoma Colo-16 cell and the expression level of EGFR. Methods: The Colo-16 cells were cultured, and then were divided into the blank control group, 5% glucose solvents control group and different concentration of oxymatrine groups (0.025 mg/mL, 0.05 mg/mL, 0.1 mg/mL, 0.5 mg/mL, 1 mg/mL and 2 mg/mL). The proliferative activity of Colo-16 cell and the expression level of EGFR and phosphorylate EGFR were detected by MTT and Western blot respectively. Results: The optical density in the different concentration of oxymatrine were lower than those in the blank control group and 5% glucose solvents control group at 24 h, 48 h and 72 h, with significant differences (P＜0.05). However, there was no significant difference of optical density between the different concentration of oxymatrine groups (P＞0.05). There was no significant difference in the levels of EGFR and phosphorylate EGFR between all the groups (P＞0.05).Conclusion: Oxymatrine can inhibit the proliferative activity of Colo-16 cell at lower concentration, in a non-concentration-dependent manner. Therefore, the effect of oxymatrine on the proliferative activity is not through inhibition of the expression of EGFR.

Key words: Oxymatrine, cutaneous squamous cell carcinoma, proliferation, EGFR